KSPublications - Time-Controlled Microfluidic Seeding in nL-Volume...

Title

Time-Controlled Microfluidic Seeding in nL-Volume Droplets to Separate Nucleation and Growth Stages of Protein Crystallization

Authors

Gerdts CJ, et al

Abstract

This paper describes a method of time-controlled seeding to
separate the stages of nucleation and growth in protein
crystallization using a microfluidic device. We quantified
microfluidic seeding using a model protein, developed a
strategy to produce diffraction-quality crystals of proteins
recalcitrant to traditional methods, and solved de novo the Xray crystal structure of Oligoendopeptidase F. Proteins are
crystallized to determine their three-dimensional structures,
to understand protein function, and aid in drug design, but
crystallization can be an unpredictable and stochastic process.

Journal

Angewandte Chemie Int. Ed Engl.

Date

12/11/2006

Link

DOI:10.1038/nature05411

Reference

Gerdts CJ, Tereshko V, Yadav MK, Dementieva I, Collart F, Joachimiak A, Stevens RC, Kuhn P, Kossiakoff A, and Ismagilov RF - "Time-Controlled Microfluidic Seeding in nL-Volume Droplets to Separate Nucleation and Growth Stages of Protein Crystallization" - Angew Chem Int Ed Engl. 2006 Dec 11;45(48):8156-60

PMID

17099920

Keyword

ATCG3D

TSRI Number

Attachments

Kuhn Lab at The Scripps Research Institute.