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Kuhn Lab at The Scripps Research Institute. > KSPublications > Enthalpy array analysis of enzymatic and binding reactions  

KSPublications: Enthalpy array analysis of enzymatic and binding reactions

Title

Enthalpy array analysis of enzymatic and binding reactions  

Authors

Recht, M.I., De Bruyker, D., Bell, A.G., Wolkin, M.V., Peeters, E., Anderson, G.B., Kolatkar, A.R., Bern, M.W., Kuhn, P., Bruce, R.H., Torres, F.E. 

Abstract

Enthalpy arrays enable label-free, solution-based calorimetric detection of molecular interactions in a 96-detector array format. The combination of the small size of the detectors and the ability to perform measurements in parallel results in a significant reduction of sample volume and measurement time compared with conventional calorimetry. We have made significant improvements in the technology by reducing the temperature noise of the detectors and improving the fabrication materials and methods. In combination with an automated measurement system, the advances in device performance and data analysis have allowed us to develop basic enzyme assays for substrate specificity and inhibitor activity. We have also performed a full titration of 18-crown-6 with barium chloride. These results point to future applications for enthalpy array technology, including fragment-based screening, secondary assays, and thermodynamic characterization of leads in drug discovery.

Journal

Analytical biochemistry 

Year

2008 

Date

4/28/2008 

Link

Link 

Reference

Recht, M.I., De Bruyker, D., Bell, A.G., Wolkin, M.V., Peeters, E., Anderson, G.B., Kolatkar, A.R., Bern, M.W., Kuhn, P., Bruce, R.H., Torres, F.E. (2008) Enthalpy array analysis of enzymatic and binding reactions. Vol. 377, 1, 33-39

PMID

18374654 

Keyword

SPI 

TSRI Number

 
Attachments
Created at 4/28/2008 10:27 AM  by Joshua Kunken 
Last modified at 4/18/2012 10:51 AM  by Katya Kadyshevskaya