Publications - "In situ data collection and structure refinement...

First Author

Yadav MK, Gerdts CJ, Sanishvili R, Smith WW, Roach LS,b Ismagilov RF, Kuhn P and Stevens RC

Title

"In situ data collection and structure refinement from microcapillary protein crystallization." J. Appl. Cryst. 38, 900-905.(2005)

Abstract

In order to decrease the time and increase the efficiency from protein crystallization hits to refined atomic models, protocols for in situ X-ray data collection from crystals grown by `microcapillary' protein crystallization techniques are being developed, through which appropriate capillary materials and fluorocarbon oils are suggested on the basis of minimizing background X-ray scattering. The methodology eliminates the need for crystal handling, cryocooling and mounting, and reduces the volume of consumed protein solution by tenfold relative to the currently available protein crystallization tools.

Link

PDF

date

12/14/2005

Authors

Attachments

Kuhn Lab at The Scripps Research Institute.